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KMID : 1007519960050020119
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Food Science and Biotechnology
1996 Volume.5 No. 2 p.119 ~ p.123
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Antioxidative Activity of Flavonoids in Persimmon Leaves
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Choi, Sang Won
Kang, Woo Won/Chung, Shin Kyo/Cheon, Sung Ho
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Abstract
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The ethylaeetate fraction obtained from the methanol extract of leaves of the persimmon Diospyros kaki Thunb., with strong antioxidative activity, was fractionated by Amberlite XAD-2 column chromatography, and the antioxidative components were separated by Sephadex LH-20 column chromatography and HPLC techniques. As a result, the isolated four flavonoid compounds such as isoquercitrin (quercetin 3-Ο-β-D-glucopyranoside), quercetin 3-Ο-β-D-glucopyranoside-2"-gallate, kaempferol 3-Ο-β-D-glucopyranoside-2"-gallate and astragalin (kaempferol 3-Ο-β-D-glucopyranoside) were identified by ¹Hand ^(13)C-NMR spectroscopy. The antioxidative activity of the four flavonoids was much higher than that of α-tocopherol. In particular, quercetin 3-Ο-β-D-glucopyranoside-2"-gallate showed the most potent inhibitory activity against the lipid peroxidation of a rabbit erythrocyte membrane ghost, and the, soybean lipoxygenase.
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